HISTOLOGY
We offer a unique pipeline from sample processing, staining (histology) and image acquisition (bioimaging) through to data analysis, providing publication-ready results.
By using histology with our advanced fluorescent microscopes and the bioimaging analysis services we offer, we can help you to understand complex cellular distributions, interactions and networks in the context of individual tissue sections or entire organs themselves.
We have extensive experience in histological techniques including Formalin-Fixed, Paraffin Embedding (FFPE) of tissue samples and cryosectioning techniques. We can provide generic histological staining such as H&E, PAS, Sirius Red and Masson Trichome and we have experience with OPAL staining for fluorescent chromogenic staining. We routinely perform multicolour direct-conjugated immunofluorescent staining on sectioned samples.
One of our goals is to develop new techniques that benefit the scientific community. We are always open to new and innovative approaches that provide a more precise and in-depth understanding of the scientific questions we are trying to address.
Below is an example of our histology and immunofluorescence staining capabilities where we performed exhaustive histological experiments to identify innate and adaptive immune infiltration inside tumour.
Treatment with intratumoural α-GalCer and CpG induces extensive infiltration of immune cells. Mice (CD45.1+) were engrafted with E.G7-OVA (CD45.2+) tumours on both flanks, and either treated intratumourally with α-GalCer and CpG (with delayed first dose) on one flank, as described for Figure 1, or with PBS according to the same schedule. tumours were collected on day 12 post tumour engraftment, 5 days after the start of treatment for analysis by immunohistochemistry. Antibody staining was against the molecular markers indicated. Average values from 10 images per section are plotted with n = 4 mice per group. Staining for CD45.1 and CD8α are shown in the top row (a-d) and staining with CD45.1 and F4/80 shown in the bottom row (e-h). Double positive cells (i.e. CD45.1+CD8α+ or CD45.1+F4/80+ cells) appear as white cells in the images. Scale bar = 50 μm. (i) Quantification of CD45+ cells per total nuclei per slide is shown. One-way ANOVA with Tukey’s multiple comparison test; *p < .05. (j) Number of CD8+ T cells (defined as CD45+CD3+CD8+) and (k) F4/80 cells (defined as CD45.1+F4/80+) per nuclei per section. (l) Frequency of voids (defined as intact 0.003–0.044 mm2 empty circular structures) per section. One-way ANOVA with Tukey’s multiple comparison test was performed. (Kef K Prasit, & Laura Ferrer-Font. OncoImmunology 2022)