We offer a unique pipeline from sample processing, staining (histology) and image acquisition (bioimaging) through to data analysis, providing publication-ready results.
By using histology with our advanced fluorescent microscopes and the bioimaging analysis services we offer, we can help you to understand complex cellular distributions, interactions and networks in the context of individual tissue sections or entire organs themselves.
We have extensive experience in histological techniques including Formalin-Fixed, Paraffin Embedding (FFPE) of tissue samples and cryosectioning techniques. We can provide generic histological staining such as H&E, PAS, Sirius Red and Masson Trichome and we have experience with OPAL staining for fluorescent chromogenic staining. We routinely perform multicolour direct-conjugated immunofluorescent staining on sectioned samples.
One of our goals is to develop new techniques that benefit the scientific community. We are always open to new and innovative approaches that provide a more precise and in-depth understanding of the scientific questions we are trying to address.
Below is an example of our histology and immunofluorescence staining capabilities where we performed exhaustive histological experiments to identify innate and adaptive immune infiltration inside tumour.
Z-stack image of fully stained whole-mount skin using six florescent dyes recorded from the dermisepidemis position showing: red (MHCII-eFluor 450), green (Lyve-1-AF488), blue (CD64-AF594), magenta (CD31-BV480), yellow (CD103-PE), cyan (CD326-AF647). (A) Maximum intensity projection image of the dermal layer (14 μm). A-1 to A-6, individual channels showing staining patterns. (B) Maximum intensity projection of epidermis (30 μm) of the sample. B-1 to B-6, individual channels showing staining pattern. Scale bar 50 μm.